en زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;"><strong><em>Background:</em></strong> The assembly and disassembly of the focal adhesions (FA) components occurs throughout&nbsp;life cycle of adhesion, with conservation of balance between removal and recruitment rate during temporal&nbsp;stages. Previous studies have demonstrated that phosphotidyilinositols play a role in regulating FA turnover.&nbsp;However, a little attention has been given to quantify the dynamics changes of Phosphatidylinositol 3,4,5-trisphosphate (PtdIns (3,4,5) P3) within and during fast and slow turnover rates of FA.<br> <br> <strong><em>Methods:</em></strong> MDA-MB-231 breast cancer cell line was used as a model in this study due to high metastatic&nbsp;and motile. These cells were co-transfected with GFP- paxillin/vinculin, as FA marker, and the&nbsp;GFP/mCherry-Btk-PH, as a biosensor to visualize PtdIns (3,4,5) P3. Confocal time-lapse images were used<br> to monitor changes or differences in the local generation of PtdIns (3,4,5) P3 within and during assembly&nbsp;and disassembly of FA. Following transfection, immunostaining was used to examine the spatial colocalization&nbsp;between FA and PtdIns (3,4,5) P3.<br> <br> <em><strong>Results: </strong></em>Our data demonstrated that PtdIns (3,4,5) P3 co-localized with FAs and increase during assembly&nbsp;and decline during disassembly of FA which exhibits slow turnover rates and was in a constant level during&nbsp;assembly and disassembly of FA that displays fast turnover rates.&nbsp;<br> <br> <em><strong>Conclusions:</strong></em> Our result suggested that the dynamic changes of PtdIns (3,4,5) P3, it may depend on&nbsp;components undergo turnover, such that early, nascent FA displays fast turnover rates and mature FA&nbsp;exhibits slow turnover rates. Thus, the local enrichment of PtdIns (3,4,5) P3 enhances FA assembly and&nbsp;disassembly activation.</div> Cancer cell migration, Focal adhesion turnover, MDA-MB-231 cell line, PtdIns (3,4,5) P3. 262 269 http://rbmb.net/browse.php?a_code=A-10-749-5&slc_lang=en&sid=1 Dhurgham Al-Fahad dhurgham.alfahad@sci.utq.edu.iq. 100319475328460012205 100319475328460012205 Yes Department of Pharmaceutical Sciences, College of Pharmacy University of Thi-Qar, Thi-Qar 64001, Iraq. Firas Alyaseen 100319475328460012206 100319475328460012206 No Department of Pharmaceutical Sciences, College of Pharmacy University of Thi-Qar, Thi-Qar 64001, Iraq. Ahmed Al-Amery 100319475328460012207 100319475328460012207 No epartment of Physiology, College of Medicine, University of Thi-Qar, Iraq. Gagandeep Singh 100319475328460012208 100319475328460012208 No Viral Research and Diagnostic Laboratory, Department of Microbiology, Osmanian Medical College, Hyderabad, Telangana, India & Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India. Mote Srinath 100319475328460012209 100319475328460012209 No Viral Research and Diagnostic Laboratory, Department of Microbiology, Osmanian Medical College, Hyderabad, Telangana, India. Yahya Abbas yahia_alkhafaji@utq.edu.iq 100319475328460012210 100319475328460012210 No Department of Biology, College of Science, University of Thi-Qar, Thi-Qar, 64001 Iraq. Hafiz Muzzammel Rehman 100319475328460012211 100319475328460012211 No School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan. Yahya Abbas 100319475328460012212 100319475328460012212 No Department of Biology, College of Science, University of Thi-Qar, Thi-Qar, 64001 Iraq.