Reports of Biochemistry and Molecular Biology

en Determination of Normal Range of Acylcarnitine in Neonatal Dried Blood Spots using LC-MS/MS بیوشیمی Biochemistry مقالات اصلی Original Article <div class="WordSection1" style="text-align: justify;">Background: Acylcarnitine is one of the crucial markers of fatty acid metabolism, and examination of their level in infants can reveal several Inherited Metabolic Disorders (IDM) or Inborn errors of Metabolism (IEM). Because of the great importance of hereditary, metabolic, and other inherited disorders early diagnosis before the appearance of clinical symptoms, this study was carried out to establish a reference range for carnitine analytes and to identify acylcarnitine profiles in normal weight neonatal dried blood spots (DBS) specimens. Methods: By using liquid chromatography tandem mass spectrometry (LC-MS/MS) for neonatal screening and eventually the examination and analysis of LC-MS/MS results, 34 acylcarnitine derivatives were identified. Results: The normal range for acylcarnitine analytes with carbon numbers ranging from zero to 18, both main and the branched ones, were ultimately measured. Afterward, they were compared with the results of some other diagnostic laboratories to be verified. Conclusion: This study differed from the other findings, which could be due to diversity in population and work methods. However, the reference range of most acylcarnitine derivatives in Tehran closely aligned with this study's findings.</div> <div style="text-align: justify;"><div aria-label="Page Break" class="cke_pagebreak" contenteditable="false" data-cke-display-name="pagebreak" data-cke-pagebreak="1" style="page-break-after:always" title="Page Break"></div></div> Acylcarnitines, Dried Blood Spot, LC-MS/MS, Newborn screening, Reference range. 522 529 http://rbmb.net/browse.php?a_code=A-10-145-2&slc_lang=en&sid=1 Jaafar Sadeq Abdulridha 100319475328460020208 100319475328460020208 Yes Department of Clinical Biochemistry, Mashhad University of Medical Sciences, Mashhad, Iran. Baratali Mashkani 100319475328460020209 100319475328460020209 No Department of Clinical Biochemistry, Mashhad University of Medical Sciences, Mashhad, Iran. Amin Alaei 100319475328460020210 100319475328460020210 No Department of Medical Laboratory Science, Varastegan Institute for Medical Sciences, Mashhad, Iran & Division of Metabolic Disorder, Pardis Clinical and Genetic Laboratory, Mashhad, Iran & Research Committee, Department of Medical Laboratory Science, Varastegan Institute for Medical Sciences, Mashhad, Iran. Mostafa Boskabadi 100319475328460020211 100319475328460020211 No School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. Abdolreza Varasteh 100319475328460020212 100319475328460020212 No Department of Medical Laboratory Science, Varastegan Institute for Medical Sciences, Mashhad, Iran & Division of Metabolic Disorder, Pardis Clinical and Genetic Laboratory, Mashhad, Iran. Fatemeh Keyfi keifyf@varastegan.ac.ir 100319475328460020213 100319475328460020213 No Department of Medical Laboratory Science, Varastegan Institute for Medical Sciences, Mashhad, Iran & Division of Metabolic Disorder, Pardis Clinical and Genetic Laboratory, Mashhad, Iran.