en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Background: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Psoriasis is a chronic inflammatory immune mediated disease arising from interaction&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">between genetic risk variants and the environment. Maternally expressed gene3 (MEG3) is a long&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">noncoding RNA (lncRNA) known for gene transcription regulation and inhibiting proliferation.&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">MEG3 competes with microRNA (miRNA-21) influencing cell proliferation and apoptosis balance.&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Endoplasmic reticulum (ER) stress proteins promote cell survival via unfolded protein response&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">(UPR) influenced by MEG3. We aimed to detect the possible role of MEG3, miRNA-21 and ER&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">stress proteins in pathogenesis of psoriasis vulgaris.</span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Methods: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Human GRP78, ATF6, caspase3 tissue levels were assayed by Enzyme Linked&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Immunosorbent Assay (ELISA). Assessment of long non-coding MEG3 and miRNA 21 expressions&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">was done by quantitative real time polymerase chain reaction (qRT-PCR).</span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Results: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">Expression of MEG3 was significantly downregulated, while miRNA-21 was remarkably&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">upregulated, ER stress proteins GRP78, ATF6, and caspase 3 all showed low levels in homogenized&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">psoriatic lesions when compared to normal skin. miRNA 21 and MEG3 were identified as possible&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">diagnostic markers for psoriasis vulgaris.</span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:TimesNewRomanPS-BoldItalicMT"><span style="color:#000000"><span style="font-weight:bold"><span style="font-style:italic">Conclusions: </span></span></span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">MEG3 is barely expressed in psoriatic lesions while miRNA-21 expression is&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">remarkably elevated but when correlated to each other there was unexpected positive correlation.&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">MEG3 and miRNA-21 were identified as possible diagnostic markers for psoriasis. Undifferentiated&nbsp;</span></span></span><span style="font-size:12pt"><span style="font-family:Times New Roman"><span style="color:#000000">psoriatic lesions have very weak UPR. </span></span></span></div> <div style="text-align: justify;"></div> ER stress proteins, lncRNA MEG3, Microrna-21, Psoriasis. 367 376 http://rbmb.net/browse.php?a_code=A-10-959-2&slc_lang=en&sid=1 Zeinab Ahmad Nour Zeinab.nour@kasralainy.edu.eg. 100319475328460016684 100319475328460016684 Yes Medical Biochemistry and Molecular Biology, Faculty of Medicine,Cairo University, Cairo, Egypt. Yasmin Elwan 100319475328460016685 100319475328460016685 No Medical Biochemistry and Molecular Biology, Faculty of Medicine,Cairo University, Cairo, Egypt. Yasser Nassar 100319475328460016686 100319475328460016686 No Medical Biochemistry and Molecular Biology, Faculty of Medicine,Cairo University, Cairo, Egypt. Maha Fathy Elmasry 100319475328460016687 100319475328460016687 No Maha Fathy Elmasry, Dermatology, Faculty of Medicine,Cairo University, Cairo, Egypt. Laila Rashed 100319475328460016688 100319475328460016688 No Medical Biochemistry and Molecular Biology, Faculty of Medicine,Cairo University, Cairo, Egypt Sara Salama Ashour 100319475328460016689 100319475328460016689 No Medical Biochemistry and Molecular Biology, Faculty of Medicine,Cairo University, Cairo, Egypt