Reports of Biochemistry and Molecular Biology

en The Use of Chitosan Nanoparticles for Delivery of CpG ODN in Treatment of Allergic Balb/C Mice بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: This study aims to prepare high stability chitosan nanoparticles (CNP) and examine the ability of CNP in CpG-ODN delivery when treating allergic mice model.  </div> <div style="text-align: justify;">Methods: Preparation and characterization of CNP were performed by ionic gelation, dynamic light scattering, and zeta sizer. The CNP cytotoxicity and activation ability of CpG ODN delivered with CNP were tested using a cell counting kit-8 and Quanti blue method. Allergic mice were injected intraperitoneal with 10 ug ovalbumin on day 0 and 7, and then treated with intranasal CpG ODN/CpG ODN, delivered with CNP/CNP, on the third week three times per week for three weeks. The ELISA method measured cytokine and IgE profiles in the allergic mice’s plasma and spleen.  </div> <div style="text-align: justify;">Results: CNP results have sizes 27.73 nm±3.67 dan 188.23 nm±53.47, spherical in shape and non-toxic, and did not alter the NF-κB activation of CpG ODN in RAW-blue cells. The application of CpG ODN delivered by chitosan nanoparticles shows no statistical difference between groups of IFN-γ, IL-10, and IL-13 in Balb/c mice’s plasma and spleen, in contrast with IgE level.</div> <div style="text-align: justify;"> Conclusions: The results showed that using chitosan nanoparticles as a delivery system for CpG ODN has the potency to safely CpG ODN efficacy.  </div> Allergy, Chitosan nanoparticle, CpG ODN, Immunotherapy, Mice spleen. 599 613 http://rbmb.net/browse.php?a_code=A-10-993-1&slc_lang=en&sid=1 Febriana Catur Iswanti febriana.iswanti@ui.ac.id 100319475328460016803 100319475328460016803 Yes Department of Biochemistry and Molecular Biology Faculty of Medicine, Universitas Indonesia, Indonesia. Qarina Hasyala Putri 100319475328460016804 100319475328460016804 No Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Indonesia. Ani Retno Prijanti 100319475328460016805 100319475328460016805 No Department of Biochemistry and Molecular Biology Faculty of Medicine, Universitas Indonesia, Indonesia. Samsuridjal Djauzi 100319475328460016806 100319475328460016806 No Department of Internal Medicine, Faculty of Medicine, Ciptomangunkusumo Hospital, Universitas Indonesia, Indonesia. Mohamad Sadikin 100319475328460016807 100319475328460016807 No Department of Biochemistry and Molecular Biology Faculty of Medicine, Universitas Indonesia, Indonesia. Arief Budi Witarto 100319475328460016808 100319475328460016808 No Department of Molecular Biology and Cell, Faculty of Medicine, Indonesia Defense University, Indonesia. Tomohiko Yamazaki 100319475328460016809 100319475328460016809 No Research Center for Functional Materials, National Institute for Materials Science (NIMS), Japan.