en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Chronic kidney disease (CKD) is a global health concern involving roughly one-tenth of developed countries&#39; populations. The flavin-containing dimethylaniline monooxygenase 3 (<i>FMO3</i>) gene encodes an enzyme that catalyzes trimethylamine N-oxide (TMAO), a toxin in CKD sufferers. This preliminary study aims to evaluate the association between coding region variations of&nbsp;<i>FMO3</i>, rs2266782G/A (E158K), rs2266780A/G (E308G), and rs1736557G/A (V257M), and the susceptibility to CKD.</span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Methods:</span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">A total of<b> </b>356 participants were enrolled, including 157 patients diagnosed with CKD and 199 age-matched healthy individuals. Genotyping of&nbsp;<i>FMO3</i>&nbsp;gene variations was performed via PCR-RFLP and ARMS-PCR methods.</span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Our findings revealed a significant association between rs2266780A/G and rs1736557G/A and CKD</span></span> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">under different genetic models. Compared to the GGG haplotype of rs2266782/rs1736557/rs2266780, the GAG, GAA, AAG, and AAA haplotype combinations conferred an increased risk of CKD in our population. Interaction analysis revealed that some genotype combinations, including GA/AA/AA, AA/AA/AA, GA/AA/GA, and GG/AG/AA, dramatically increased CKD risk in the Iranian population. No correlation was found between <i>FMO3</i> polymorphisms and CKD stages.</span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><strong><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusions:</span></span></i></strong> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">These observations highlight the potential impact of coding variants of the&nbsp;<i>FMO3</i>&nbsp;gene on the onset of CKD. Further investigations into expanded populations and diverse races are needed to confirm our findings.</span></span></span></span></span></span></div> Keywords: Chronic kidney disease, FMO3, Genetic variant, Single-nucleotide polymorphism, Trimethylamine N-oxide. 430 439 http://rbmb.net/browse.php?a_code=A-10-1001-1&slc_lang=en&sid=1 Ismail Shorudi Dadi 100319475328460016712 100319475328460016712 No Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran & Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Ramin Saravani 100319475328460016713 100319475328460016713 No Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran & Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Tahereh Khalili taherehkh2131@yahoo.com 100319475328460016714 100319475328460016714 Yes Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran & Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Saman Sargazi* sgz.biomed@gmail.com. 100319475328460016715 100319475328460016715 No 2: Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Mahdi Majidpour 100319475328460016716 100319475328460016716 No Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran & Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Mohammad Sarhadi 100319475328460016717 100319475328460016717 No Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Shekoufeh Mirinejad 100319475328460016718 100319475328460016718 No Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Sheida Shahraki 100319475328460016719 100319475328460016719 No Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Ali Alidadi 100319475328460016720 100319475328460016720 No Department of Nephrology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran & Clinical Immunology Research Center (CIRC), Zahedan University of Medical Sciences, Zahedan, Iran.