en زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Background:</span></span></span></i></b><b><i> </i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Cancer continues worldwide. It has been reported that OTUB1, a cysteine protease, plays a critical role in a variety of tumors and is strongly related to tumor proliferation, migration, and clinical prognosis by its functions on deubiquitination. Drug advances continue against new therapeutic targets. In this study we used OTUB1 to develop a specific pharmacological treatment to regulate deubiquitination by OTUB1. The aim of this research is to regulate OTUB1 functions.</span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Methods:</span></span></span></i></b> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">By molecular docking in a specific potential OTUB1 interaction site between Asp88, Cys91, and His26 amino acids, using a chemical library of over 500,000 compounds, we selected potential inhibitors of the OTUB1 catalytic site.</span></span></span><br> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt"></span></span></span></span></span></span></span><br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Results:</span></span></span></i></b> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Ten compounds (OT1 - OT10) were selected by molecular docking to develop a new anti-cancer drug to decrease OTUB1 functions in cancer processes.</span></span></span><br> <span lang="EN-ID" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt"></span></span></span></span></span></span></span><br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Conclusions:</span></span></span></i></b> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">OT1 &ndash; OT10 compounds could be interacting in the potential site between Asp88, Cys91, and His265 amino acids in OTUB1. This site is necessary for the deubiquitinating function of OTUB1. Therefore, this study shows another way to attack cancer.</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt"></span></span></span></span></span></span></span></div> Anti-cancer, Dub inhibitor, Molecular docking, OTUB1. 684 693 http://rbmb.net/browse.php?a_code=A-10-1044-1&slc_lang=en&sid=1 Octavio Galindo-Hernández 100319475328460016853 100319475328460016853 No Autonomous University of Baja California, School of Medicine Campus Mexicali, Mexicali, BC, México. Lizbeth Angelina García-Salazar 100319475328460016854 100319475328460016854 No Autonomous University of Baja California, School of Medicine Campus Mexicali, Mexicali, BC, México. Victor Guadalupe García-González 100319475328460016855 100319475328460016855 No Autonomous University of Baja California, School of Medicine Campus Mexicali, Mexicali, BC, México. Raúl Díaz-Molina 100319475328460016856 100319475328460016856 No Autonomous University of Baja California, School of Medicine Campus Mexicali, Mexicali, BC, México. José Luis Vique-Sánchez jvique@uabc.edu.mx 100319475328460016857 100319475328460016857 Yes Autonomous University of Baja California, School of Medicine Campus Mexicali, Mexicali, BC, México.